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Treatment with ferulic acid to rats with streptozotocin-induced diabetes: effects on oxidative stress, pro-inflammatory cytokines, and apoptosis in the pancreatic cell

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The supernatant was discarded, 600 L of 75% ethanol (anhydrous ethanol:DEPC-treated water = 3:1) was added, the precipitate was oscillated, and the precipitate was washed and centrifuged at 12,000 rpm at 4 for 5 min (this process was repeated 3 times)